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Hair Protein Quantification

Immunohistochemistry and ELISA Methods

Epistem has reliable and reproducible techniques for precise protein quantification in single plucked human hairs through immunohistochemistry and ELISA methods. These techniques allow for the detection and quantification of protein expression and site-specific phosphorylation, with customisable labelling and quantification options available for each antigen.

An Overview

Utilising Immunohistochemistry and ELISA Methods for Protein Quantification in Human Hair Samples

Epistem can provide techniques for protein quantification in single plucked human hairs represent a significant advancement in the field of biomedical research. Immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) methods are used to detect and quantify protein expression and site-specific phosphorylation in hair samples. IHC is a technique used to visualise the distribution and localisation of specific antigens in tissues, including hair follicles, by using antibodies that bind to the target protein and produce a visible signal. ELISA, on the other hand, is a biochemical assay that detects and measures the presence of proteins in a sample, often using a colorimetric readout.

Using either whole mount or longitudinal sectioning techniques, Epistem’s methods enable the accurate and precise quantification of protein expression and site-specific phosphorylation in hair samples. Additionally, customised labelling protocols and quantification methods can be developed for each antigen of interest, allowing for a more comprehensive analysis of protein function in hair follicles.

Epistem’s ex vivo culture technique enables direct engagement of therapeutic target pathways, which can be demonstrated at the preclinical stage in their Good Clinical Laboratory Practice (GCLP)-compliant histology laboratories. This technique involves culturing hair follicles in a controlled environment, which allows for the manipulation of specific pathways and the evaluation of potential therapeutic interventions. By translating this preclinical data into the design of clinical studies, Epistem’s techniques provide a reliable and informative basis for future therapeutic development.

Overall, Epistem’s techniques for protein quantification in single plucked human hairs using IHC and ELISA methods, combined with ex vivo culture techniques, provide a highly sensitive and precise means of analysing protein function in hair follicles. This has significant implications for our understanding of disease mechanisms and the development of new therapeutic interventions.

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