Skip to content

In Vitro Wound Healing

Rapid Mechanistic Data for Novel Therapeutic Agent Testing

Epistem provides in vitro wound healing assays that quickly generate mechanistic data to assess the impact of new therapeutic agents on wound healing processes such as cell proliferation, migration, wound contraction, and angiogenesis. The assays can be performed using cell lines, primary human or murine keratinocytes, and primary human or murine fibroblasts. Upon request, primary cells can be provided from different donor types and ages or other species.

Cell Proliferation and Migration Contraction Assays

Discover how Epistem's in vitro wound healing assays can accelerate your wound healing research by providing rapid, reliable, and detailed mechanistic data on cell proliferation, migration, and contraction processes.

Cell Proliferation and Migration Assays

The rapid closure of open wounds by the proliferation and migration of epidermal cells is vital to reduce the risk of infection and further complications. These assays can be used to screen for novel therapeutic agents that promote wound closure.

Scratch Assay – Monolayers of confluent cells are mechanically scratched and the rate at which the cells migrate to close the scratch is measured. Simultaneous analysis of effects on proliferation and migration can be performed.

High-Throughput Migration Assay – Monolayers of cells are seeded into 96-well plates, with stoppers in the centre of each well creating a void. Stoppers are removed and the rate of cell migration into the void is quantified. Effects on proliferation can be measured simultaneously and used to determine the relationship with cell migration.

3D Migration Assay – A collagen window is created around a spheroid or dense ball of fibroblasts and suspended in media. Cell migration out from this ball of cells is calculated as a percentage of the original area.

Wound Contraction Assay

Promoting fibroblast contraction reduces the size of an open wound, speeding up the healing process. Epistem’s Wound Contraction Assay can be used to screen for the effect of novel therapeutic agents on wound contraction.

Fibroblasts are seeded into collagen gels and the novel therapeutic agent applied. Contraction typically occurs over the following 5 days. Gels are imaged and the effect of novel therapeutic agent on the rate of contraction determined.

Download the latest brochure

From brochures to posters, find all the information you need in a downloadable & printable format.

Contact Information

For more information regarding our services, please do not hesitate to contact us.