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Leukaemic Orthotopic Models

Epistem offers acute myeloid leukaemia (AML) & acute lymphoblastic leukaemia (ALL) orthotopic models

Cell lines are lentivirally transduced with luciferase and GFP, enabling disease progression and response to novel therapeutic agents to be monitored longitudinally using Epistem's state-of-the-art imaging facility. All of our models are fully complemented by our range of in vitro and ex vivo assays, as well as our comprehensive FACS, histology and biomarker analytical services.

Advanced Orthotopic Models for Acute Leukaemia Research

Epistem offers reliable and clinically relevant orthotopic models for AML and ALL, enabling researchers to study disease progression and evaluate potential therapeutics.

Acute Myeloid Leukaemia

Acute Myeloid Leukaemia is the most common form of adult leukaemia and is characterised by clonal proliferation of myeloid precursors. Human leukemic cell lines are intrafemorally or intravenously injected to ensure consistent engraftment and development of disease at the clinically applicable site. Intravenous injection of leukemic cells is also available.

Epistem’s orthotopic Acute Myeloid Leukaemia model is available with MV4-11 cell lines which have a t(8:21) (AML-ETO) translocation representative of approximately 25% of AML patients. These cell lines have been lentivirally transduced with luciferase and GFP, allowing in-life disease monitoring using optical imaging.

Cells expressing luciferase and GFP are transplanted intrafemorally. Engraftment can be consistently detected by optical imaging at an early stage of disease, dependent on the growth kinetics of the cell line. Solid tumour formation, as seen in some AML patients, is observed alongside limited systemic disease.

Acute Lymphoblastic Leukaemia

Epistem offers orthotopic models of Acute Lymphoblastic Leukaemia (ALL). Human leukemic cell lines are intrafemorally injected to ensure consistent engraftment and development of disease at the clinically applicable site. Intravenous injection of leukemic cells is also available.

Epistem’s orthotopic Acute Lymphoblastic Leukaemia model uses the SEM cell line. The karyotype of the SEM cell line is most common in children under 12 months of age and is characterised by the overproduction and lack of differentiation of lymphocytes.

Cells (SEM) expressing luciferase and GFP are transplanted intrafemorally. Engraftment is detected by optical imaging on average, by day 7 and systemic disease can be typically observed by day 14.

For solid tumour orthotopic models, click here.

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