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IL-10 Knockout Spontaneous Colitis

Advancing Colitis Research with IL-10 Knockout Mouse Models: A Robust Tool for Investigating Disease Pathogenesis and Therapeutic Interventions

Epistem's IL-10 knockout mouse models for spontaneous colitis research offer a reliable and cost-effective tool for investigating disease pathogenesis and developing novel therapeutic interventions.

Explore the Versatile Applications and Benefits of IL-10 Knockout Mice in Colitis Research

Using IL-10 knockout research models provides a valuable approach to investigating the potential pathogenesis of spontaneous colitis and developing effective therapeutic strategies for the disease.

IL-10 is a cytokine that plays a critical role in regulating the immune system and inflammation. IL-10 knockout mice develop colitis spontaneously over time, unless they are maintained under germ-free conditions.

Without IL-10, the immune response in these mice becomes dysregulated, resulting in chronic inflammation and the development of colitis. Similar to the adoptive T-cell transfer model, IL-10 knockout mice exhibit a Th1/Th17-type immune response and show features such as epithelial hyperplasia, mixed inflammatory cell infiltrate in the mucosa and submucosa, crypt abscess formation, and occasional ulceration. Additionally, these mice may exhibit the formation of cryptopatches, which are lymphoid aggregates found within the intestinal mucosa that are thought to play a role in the immune response.

By using IL-10 knockout spontaneous colitis research models, researchers can better understand the pathogenesis of colitis and develop effective therapeutic strategies for treating inflammatory bowel diseases.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Diarrhoea severity
  • Weight loss
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression
  • Intestinal permeability assay (FITC Dextran)
  • Fecal Lipocalin (Lcn-2) assay

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